TEM8 Rabbit Polyclonal Antibody

SKU: orb158588

Description

TEM8 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TEM8 (201-300/564aa)
• Target: ANTXR1
• Molecular Weight: 63 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Anthrax toxin receptor 1; Anthrax toxin receptor 1 precursor; ANTXR 1; ANTXR1; ATR; TEM-8; TEM 8; Tumor Endothelial Marker 8; Tumor endothelial marker 8 precursor; ANTR1_HUMAN; Anthrax toxin receptor-1; FLJ11298; FLJ21776.

Sample: HepG2 (Human) Cell Lysate at 30 ug, K562 (Human) Cell Lysate at 30 ug, Primary: Anti-TEM8 (orb158588) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 59 kD, Observed band size: 62 kD.

Sample: Lane 1: Human A549 cell lysates, Lane 2: Human HepG2 cell lysates, Lane 3: Human SH-SY5Y cell lysates, Lane 4: Human Hela cell lysates, Lane 5: Human U2os cell lysates, Primary: Anti-TEM8 (orb158588) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 63 kD, Observed band size: 61 kD.

Sample: Siha (Human) Cell Lysate at 30 ug, Uterus (Mouse) Lysate at 40 ug, Primary: Anti-TEM8 (orb158588) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 59 kD, Observed band size: 62 kD.

Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TEM8) Polyclonal Antibody, Unconjugated (orb158588) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

ANTXR1

UniProt

Q9H6X2