TEAD1 Antibody

• Catalog Number: orb1260451
• Category: Antibodies
• Description: TEAD1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, IF, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 135-215 of human TEAD1 (NP_068780.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200CHIP: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 48kDa
• Target: TEAD1
• UniProt ID: P28347
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: TEAD1, AA, REF1, TCF13, TEF-1, NTEF-1, TCF-13, TEA
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200CHIP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using TEAD1 antibody (orb1260451) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.

Immunohistochemistry of paraffin-embedded human lung cancer using TEAD1 antibody (orb1260451) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using TEAD1 antibody (orb1260451) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using TEAD1 antibody (orb1260451) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human esophageal cancer using TEAD1 antibody (orb1260451) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using TEAD1 antibody (orb1260451) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using TEAD1 antibody (orb1260451) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of MCF7 cells using TEAD1 antibody (orb1260451).

Chromatin immunoprecipitation analysis of extracts of HeLa cell line, using TEAD1 rabbit polyclonal antibody (orb1260451) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.