TEAD1 Antibody

SKU: orb1260451

Description

TEAD1 Antibody

Research Area

Cell Biology, Epigenetics & Chromatin

Images & Validation

• Tested Applications: ChIP, IF, IHC, WB
• Reactivity: Human, Mouse
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200CHIP: 1:20 - 1:50

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 135-215 of human TEAD1 (NP_068780.2).
• Target: TEAD1
• Molecular Weight: Observed: 48kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

TEAD1, AA, REF1, TCF13, TEF-1, NTEF-1, TCF-13, TEAD-1

Western blot analysis of extracts of various cell lines, using TEAD1 antibody (orb1260451) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.

Immunohistochemistry of paraffin-embedded human lung cancer using TEAD1 antibody (orb1260451) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using TEAD1 antibody (orb1260451) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using TEAD1 antibody (orb1260451) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human esophageal cancer using TEAD1 antibody (orb1260451) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using TEAD1 antibody (orb1260451) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using TEAD1 antibody (orb1260451) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of MCF7 cells using TEAD1 antibody (orb1260451).

Chromatin immunoprecipitation analysis of extracts of HeLa cell line, using TEAD1 rabbit polyclonal antibody (orb1260451) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Quick Database Links

Gene Symbol

TEAD1

UniProt

P28347