TDP43 Antibody

• Catalog Number: orb1525227
• Category: Antibodies
• Description: TDP43 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 150 and 200
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: TDP43
• UniProt ID: Q13148
• NCBI: NP_031401.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: TAR DNA-binding protein-43; TAR DNA-binding protei
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse TDP43 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , Jurkat (J; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-TDP43 antibody orb1525227 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. TDP43 was also immunoprecipitated by rabbit anti-TDP43 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Detection of human TDP43 by immunohistochemistry.Sample: FFPE section of human non-small cell lung cancer. Antibody: Affinity purified rabbit anti-TDP43 (Cat. No. orb1525227) used at a dilution of 1: 1, 000 (0.2µg/ml). Detection: DAB

Detection of mouse TDP43 by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-TDP43 (Cat. No. orb1525227) used at a dilution of 1: 200 (1µg/ml). Detection: DAB