TCHP Rabbit Polyclonal Antibody

• Catalog Number: orb100712
• Category: Antibodies
• Description: TCHP Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Porcine, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TCHP (401-498/498aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 55 kDa
• Target: TCHP
• UniProt ID: Q9BT92
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: TCHP; Trichoplein keratin filament-binding protein
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Joel H Nyström 1, Taina R H Heikkilä 1, Keshav Thapa 1 2, Ilari Pulli 1, Kid Törnquist 1 3, Diana M Toivola Colonocyte keratins stabilize mitochondria and contribute to mitochondrial energy metabolism Am J Physiol Gastrointest Liver Physiol, (2024)

PubMed: 38860856

Applications

WB

Reactivity

Mouse

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TCHP) Polyclonal Antibody, Unconjugated (orb100712) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.