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TCF4 Antibody
SKU: orb413783
Description
Images & Validation
−Item 1 of 2
| Tested Applications | IHC, WB |
|---|---|
| Dilution range | WB (1:1000); IHC (1:50) |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide from the C-terminal of Human TCF4 (500-600 aa), conjugated to Keyhole Limpet Haemocyanin (KLH). |
| Target | TCF4 |
| Molecular Weight | 71 kDa, 45 kDa |
| Purification | Peptide Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−TCF-4, MGC149723, PTHS, Class B basic helix-loop-helix protein 19, bHLHb19, SEF 2, SL3 3 enhancer factor 2, SEF2 1, ITF-2, E2 2, SEF2 1A, BHLHB19, MGC149724, ITF 2, Transcription factor 4, TCF 4, SEF-2, SEF2 1B, Immunoglobulin transcription factor 2, SL3-3 enhancer factor 2, SEF2, ITF2, ITF2_HUMAN
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Immunohistochemistry analysis using Rabbit Anti-TCF4 Polyclonal Antibody. Tissue: Kidney. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-TCF4 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
Western blot analysis of Mouse liver lysate showing detection of ~71.3 kDa TCF4 protein using Rabbit Anti-TCF4 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse liver lysate. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TCF4 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~71.3 kDa. Other Band (s): ~45 kDa isoform.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001077431.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
TCF4 Antibody (orb413783)
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