TCF4 Antibody (FITC)

SKU: orb413795

Description

Rabbit polyclonal antibody against TCF4 conjugated to FITC

Images & Validation

• Tested Applications: IHC, WB
• Dilution range: WB (1:1000); IHC (1:50)
• Reactivity: Human, Mouse
• Application Notes:
  A 1:1000 dilution of SPC-723 was sufficient for detection of TCF4 in 15 µg of mouse liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from the C-terminal of Human TCF4 (500-600 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
• Target: TCF4
• Molecular Weight: 71 kDa, 45 kDa
• Purification: Peptide Affinity Purified
• Conjugation: FITC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

TCF-4, MGC149723, PTHS, Class B basic helix-loop-helix protein 19, bHLHb19, SEF 2, SL3 3 enhancer factor 2, SEF2 1, ITF-2, E2 2, SEF2 1A, BHLHB19, MGC149724, ITF 2, Transcription factor 4, TCF 4, SEF-2, SEF2 1B, Immunoglobulin transcription factor 2, SL3-3 enhancer factor 2, SEF2, ITF2, ITF2_HUMAN

Immunohistochemistry analysis using Rabbit Anti-TCF4 Polyclonal Antibody. Tissue: Kidney. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-TCF4 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Western blot analysis of Mouse liver lysate showing detection of ~71.3 kDa TCF4 protein using Rabbit Anti-TCF4 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse liver lysate. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TCF4 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~71.3 kDa. Other Band (s): ~45 kDa isoform.

Quick Database Links

Gene Symbol

TCF4

UniProt

P15884

NCBI Gene ID

6925

RefSeq (Protein):NP_001077431.1