TCF25 Antibody

• Catalog Number: orb1266965
• Category: Antibodies
• Description: TCF25 Antibody
• Target: TCF25
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit Ig
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Immunogen: This TCF25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-215 amino acids from the N-terminal region of human TCF25.
• UniProt ID: Q9BQ70
• MW: 77 kDa
• Tested applications: IF, WB
• Application notes: For IF starting dilution is: 1:10~50For WB starting dilution is: 1:1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Transcription factor 25, TCF-25, Nuclear localized
• Note: For research use only
• NCBI: Q9BQ70

Fluorescent image of A549 cell stained with TCF25 Antibody (N-term). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TCF25 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml). TCF25 immunoreactivity is localized to Cytoplasm significantly.

Western blot analysis in CEM cell line lysates (35 ug/lane).