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Catalog Number | orb1266965 |
---|---|
Category | Antibodies |
Description | TCF25 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Predicted Reactivity | Mouse |
Reactivity | Human |
Isotype | Rabbit Ig |
Immunogen | This TCF25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-215 amino acids from the N-terminal region of human TCF25. |
Concentration | batch dependent |
Dilution range | For IF starting dilution is: 1:10~50For WB starting dilution is: 1:1000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 77 kDa |
Target | TCF25 |
UniProt ID | Q9BQ70 |
NCBI | Q9BQ70 |
Storage | Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Transcription factor 25, TCF-25, Nuclear localized Read more... |
Note | For research use only |
Application notes | For IF starting dilution is: 1:10~50For WB starting dilution is: 1:1000 |
Expiration Date | 12 months from date of receipt. |
Fluorescent image of A549 cell stained with TCF25 Antibody (N-term). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TCF25 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml). TCF25 immunoreactivity is localized to Cytoplasm significantly.
Western blot analysis in CEM cell line lysates (35 ug/lane).
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