TCF21 Rabbit Polyclonal Antibody

• Catalog Number: orb574662
• Category: Antibodies
• Description: Rabbit polyclonal antibody to TCF21
• Target: TCF21
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TCF21
• Protein Sequence: Synthetic peptide located within the following region: SNCENGSPQKGRGGLGKRRKAPTKKSPLSGVSQEGKQVQRNAANARERAR
• UniProt ID: O43680
• MW: 20 kDa
• Tested applications: IHC, WB
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: POD1, bHLHa23
• Note: For research use only
• NCBI: NP_003197

25 ug of the indicated Human whole cell extracts was loaded onto a 10-20% SDS-PAGE gel. 2 ug/ml of the antibody was used in this experiment.

Sample Tissue: Human 293T Whole Cell, Antibody dilution: 1 ug/ml.

Sample Tissue: Human HCT116 Whole Cell, Antibody dilution: 3 ug/ml.

Sample Tissue: Human Hela, Antibody dilution: 1.0 ug/ml.

Sample Tissue: Human Jurkat Whole Cell, Antibody dilution: 1 ug/ml.

Sample Tissue: Human Ovary Tumor, Antibody dilution: 1 ug/ml.

Sample Tissue: Human Ovary Tumor, Antibody dilution: 1 ug/ml.

Sample Type: Hela Whole cell lysates, Antibody dilution: 1.0 ug/ml.

Positive control (+): Human kidney (KI), Negative control (-): Human liver (LI), Antibody concentration: 1 ug/ml.

Immunohistochemistry with Human Brain, cortex, senile plaque tissue at an antibody concentration of 5.0 ug/ml using anti-TCF21 antibody (orb574662).

WB Suggested Anti-TCF21 Antibody Titration: 0.2-1 ug/ml, Positive Control: Human Lung.