TCF12 Rabbit Polyclonal Antibody

SKU: orb158579

Description

TCF12 Rabbit Polyclonal Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Rat
• Predicted Reactivity: Equine, Human, Mouse, Rabbit, Sheep, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TCF12 (51-150/682aa)
• Target: TCF12
• Molecular Weight: 73 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CRS3; HEB; HH26; HTF4; HsT17266; TCF-12; bHLHb20; p64; HTF4_HUMAN; TCF12; Class B basic helix-loop-helix protein 20 (bHLHb20); DNA-binding protein HTF4; E-box-binding protein; Transcription factor HTF-4;

Paraformaldehyde-fixed, paraffin embedded (Rat colon), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (TCF12) Polyclonal Antibody, Unconjugated (orb158579) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Quick Database Links

Gene Symbol

TCF12

UniProt

Q99081