Tbx21 Rabbit Polyclonal Antibody

• Catalog Number: orb7075
• Category: Antibodies
• Description: Tbx21 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Tbx21 (201-300/535aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 59 kDa
• Target: TBX21
• UniProt ID: Q9UL17
• RRID: AB_10931734
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: T bet; T box 21; T box expressed in T cells; T box
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Lee, Soon-Young et al. Camellia japonica oil suppressed asthma occurrence via GATA-3 & IL-4 pathway and its effective and major component is oleic acid Phytomedicine, 57, 84-94 (2019)

PubMed: 30668326

Applications

IF

Reactivity

Mouse

Soon-Young Lee, Chulyung Choi, Seung-Sik Cho, Min-Hee Kim, Juyeon Park, Yongbum Kwon, Jin Woo Park, Dae-Hun Park Saururus chinensis water-extract effectively controls asthma by ameliorating of Th1/Th2 imbalance and suppressing of NF-κB/COX-2/PGE2-related inflammation JFF, 112, 105930 (2024)

PubMed:

Applications

IF

Reactivity

Mouse

Lee, Soon-Young et al. Macmoondongtang modulates Th1-/Th2-related cytokines and alleviates asthma in a murine model PLoS ONE, 14, e0224517 (2019)

PubMed: 31790411

Applications

IF

Reactivity

Mouse

Sample: Lane 1: Lung (Mouse) Lysate at 40 ug, Lane 2: Thymus (Mouse) Lysate at 40 ug, Primary: Anti-Tbx21 (orb7075) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 58 kD, Observed band size: 60 kD.

Sample: Thymus (Mouse) Lysate at 40 ug, Primary: Anti-Tbx21 (orb7075) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 59 kD, Observed band size: 59 kD.

Tissue/Cell: human gastric carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Tbx21/T-bet Polyclonal Antibody, Unconjugated (orb7075) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.