TBX21 Antibody (N-term)

• Catalog Number: orb1935818
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB34410
• Tested applications: IF, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:1000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 58328 Da
• Target: This TBX21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-50 amino acids from the N-terminal region of human TBX21.
• UniProt ID: Q9UL17
• NCBI: NP_037483.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: T-box transcription factor TBX21, T-box protein 21
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

TBX21 Antibody (N-term) western blot analysis in A549 cell line lysates (35 ug/lane). This demonstrates the TBX21 antibody detected the TBX21 protein (arrow).

Fluorescent image of A549 cell stained with TBX21 Antibody (N-term). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX21 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). TBX21 immunoreactivity is localized to Nucleus significantly.