TBX18 Rabbit Polyclonal Antibody

• Catalog Number: orb1289
• Category: Antibodies
• Description: TBX18 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TBX18 (201-300/607aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 65 kDa
• Target: TBX18
• UniProt ID: O95935
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: T box 18; T box protein 18; T box transcription fa
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (mouse embryo), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TBX18) Polyclonal Antibody, Unconjugated (orb1289) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: HT29 Cell lysate at 30 ug, Primary: Anti-TBX18 (orb1289) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 65 kD, Observed band size: 65 kD.

Sample: line 1: Mouse embryo lysate at 45 ug, line 2: Mouse brain lysate at 45 ug, Primary: Anti-TBX18 (orb1289) at 1:200 dilution, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:3000 dilution, Predicted band size: 65kD, Observed band size: 67kD, We are unsure as to the identity of these extra bands.