TBX1 Rabbit Polyclonal Antibody

SKU: orb2417

Description

TBX1 Rabbit Polyclonal Antibody

Research Area

Developmental Biology, Neurodegenerative Disease, Neuroscience

Images & Validation

• Tested Applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=2ug/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TBX1 (165-270/398aa)
• Target: TBX1
• Molecular Weight: 43 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

CAFS; CTHM; DGCR; DGS; DORV; T box 1; T box 1 transcription factor; T box 1 transcription factor C; T box; T box protein 1; T box transcription factor TBX 1; T box transcription factor TBX1; T-box 1; T-box protein 1; T-box transcription factor TBX1; TBX 1; TBX 1C; tbx1; TBX1_HUMAN; TBX1C; Testis specific T box protein; Testis-specific T-box protein; TGA; VCFS.

Blank control: K562. Primary Antibody (green line): Rabbit Anti-TBX1 antibody (orb2417), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Brain (Rat) Lysate at 40 ug, Primary: Anti-TBX1 (orb2417) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 43 kD, Observed band size: 44 kD.

Sample: Lane 1: Mouse Heart tissue lysates, Lane 2: Rat Heart tissue lysates, Lane 3: Human K562 cell lysates, Lane 4: Human 293T cell lysates, Primary: Anti-TBX1 (orb2417) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kDa, Observed band size: 43 kDa.

Sample: Muscle (Mouse) Lysate at 40 ug, Primary: Anti-TBX1 (orb2417) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kD, Observed band size: 43 kD.

Quick Database Links

Gene Symbol

TBX1

UniProt

O43435