TBX1 Antibody (C-term)

• Catalog Number: orb1927949
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB22154
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, IHC-P: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 43133 Da
• Target: This TBX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 327-356 amino acids from the C-terminal region of human TBX1.
• UniProt ID: O43435
• NCBI: NP_542377.1, NP_542378.1, NP_005983.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: T-box transcription factor TBX1, T-box protein 1,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

TBX1 Antibody (C-term) western blot analysis in HLVEC cell line lysates (35 ug/lane).This demonstrates the TBX1 antibody detected the TBX1 protein (arrow).

Western blot analysis of lysate from HLVEC cell line, using TBX1 Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug.

Immunohistochemical analysis of paraffin-embedded H. breast section using TBX1 Antibody (C-term). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Formalin-fixed and paraffin-embedded human testis tissue reacted with TBX1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Fluorescent confocal image of A2058 cell stained with TBX1 Antibody (C-term). A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX1 primary antibody (1: 25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TBX1 immunoreactivity is localized to nucleus significantly.