TBL1X/SMAP55 Rabbit Polyclonal Antibody

SKU: orb4567

Description

TBL1X/SMAP55 Rabbit Polyclonal Antibody

Research Area

Cell Biology, Coactivators and Corepressors, DNA and RNA Research, Lipid Metabolism, Lipids and Lipoproteins Metabolism, Metabolism

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Human, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TBL1X (501-577/577aa)
• Target: TBL1X
• Molecular Weight: 63 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TBL1; EBI; F box like/WD repeat protein TBL1X; SMAP 55; SMAP55; TBL 1; TBL1X; Transducin(beta) like 1; Transducin(beta) like 1 X linked; Transducin beta like 1 X linked; Transducin beta like 1X; Transducin beta like 1X protein; TBL1X_HUMAN; F-box-like/WD repeat-containing protein TBL1X; Full=Transducin beta-like protein 1X; Transducin-beta-like protein 1, X-linked..

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TBL1X) Polyclonal Antibody, Unconjugated (orb4567) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with BARD1Polyclonal Antibody, Unconjugated (orb4567) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Sample: Uterus (Mouse) Lysate at 40 ug, Primary: Anti-SMAP55 (orb4567) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 63 kD, Observed band size: 63 kD.

Quick Database Links

Gene Symbol

TBL1X

UniProt

O60907