TAZ Rabbit Polyclonal Antibody

SKU: orb158573

Description

TAZ Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=3ug/test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TAZ (1-100/400aa)
• Target: WWTR1
• Molecular Weight: 44 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Transcriptional co activator with PDZ binding motif; Transcriptional coactivator with PDZ binding motif; Transcriptional coactivator with PDZ-binding motif; WW domain containing transcription regulator 1; WW domain containing transcription regulator protein 1; WW domain-containing transcription regulator protein 1; WWTR 1; WWTR1; WWTR1_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (mouse placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TAZ) Polyclonal Antibody, Unconjugated (orb158573) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Blank control: A549. Primary Antibody (green line): Rabbit Anti-TAZ antibody (orb158573), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 3 µg/Test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TAZ) Polyclonal Antibody, Unconjugated (orb158573) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: Kidney (Mouse) Lysate at 40 ug, Lane 2: Kidney (Rat) Lysate at 40 ug, Primary: Anti-TAZ (orb158573) at 1/1000 dilution, Anti-beta-Actin (orb500817) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50-55 kD, Observed band size: 52 kD.

Sample: MCF-7 (Human) Cell Lysate at 40 ug, Lovo (Human) Cell Lysate at 40 ug, A549 (Human) Cell Lysate at 40 ug, Primary: Anti-TAZ (orb158573) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 44kD, Observed band size: 46kD.

Quick Database Links

Gene Symbol

WWTR1

UniProt

Q9GZV5

References

Sun, Lidan et al. Prognostic impact of TAZ and β-catenin expression in adenocarcinoma of the esophagogastric junction Diagn Pathol, 9, 125 (2014)

PubMed: 25029906