Tau Rabbit Polyclonal Antibody

SKU: orb1294394

Description

Rabbit polyclonal antibody to Tau

Research Area

Neuroscience

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: Western Blot 1:3000 – 1:5000, Immunofluorescence 1:200 – 1:500, Immunohistochemistry (Paraffin) 1:200 – 1:300, Immunohistochemistry (Frozen) 1:200 – 1:400
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Target: Tau
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

All lanes: Anti-Tau antibody Lysates/proteins at 45 µg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294394 overnight at 4°C.

Anti-Tau antibody Lysates at 40 µg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294394 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294394 anti-Tau antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunofluorescent analysis. Sample: primary cortical neurons, Tau antibody (orb1294394): 1-300, Anti-rabbit 488: 1-500, Fixed: 4% paraformaldehyde at RT for 20 min.

Immunofluorescent analysis. Sample: primary cortical neurons, Tau antibody (orb1294394): 1-300, Anti-rabbit 488: 1-500, Fixed: 4% paraformaldehyde at RT for 20 min.

Immunohistochemical analysis of paraffin embedded Human astroglioma tissue labeling Tau with orb1294394 at 1/300.

Immunohistochemical analysis of paraffin embedded Human brain tissue labeling Tau with orb1294394 at 1/200.

Immunohistochemical analysis of paraffin embedded mouse Alzheimer’s tissue labeling Tau with orb1294394 at 1/1000 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical of frozen sections. Sample: mouse cerebellum. Green: Tau antibody (orb1294394): 1-300, Anti-rabbit 488: 1-500, DAPI (blue) was used as the nuclear counter stain.

Quick Database Links

Gene Symbol

Tau