TaqDNA Protein

SKU: orb90008

Description

Recombinant Taq DNA Polymerase protein

Images & Validation

• Application Notes:
  Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25°C), 50mM NaCl, 5mM MgCl2, 200µm each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), 10µg activated calf thymus DNA and 0.1mg/ml BSA in a final volume of 50ul. Storage Buffer: Compatibility with Reaction Buffers: Taq DNA Polymerase in Storage Buffer. Use of other reaction buffers that do not contain Triton X-100 (final concentration of 0.1%) will result in inactivation of the enzyme. 50mM Tris-HCl (pH 8.0), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 50% glycerol and 1% Triton X-100. 10X Reaction Buffer with MgCl2: 500mM KCl, 100mM Tris-HCl (pH 9.0 at 25C), 1% Triton X-100 and 15mM MgCl2. Buffer is optimized for use with 0.2mM of each dNTP

Key Properties

• Source: Recombinant e.coli contains Thermus aquaticus polymerase gene
• Purity: Greater than 95.0% as determined by SDS-PAGE.

Storage & Handling

• Storage: Stability: Stable for 5 days at 10°C, for longer period of time store at -20°C
• Buffer/Preservatives: Taq DNA Polymerase solution in 20mM Tris-HCl, pH 8.0, 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.5% NP40, 0.5% Tween 20.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA polymerase I thermostable, EC 2.7.7.7, Taq polymerase 1.