Taq Plus DNA Protein

SKU: orb90007

Description

Recombinant Taq Plus DNA Polymerase protein

Images & Validation

• Application Notes:
  Unit Definition: One unit of the enzyme catalyzes the incorporation of 10nmole of deoxyribonucleotides into a polynucleotide fraction in 30 min at 74°C. Note: All reagents, including Taq Plus, should be mixed immediately before use. Storage Buffer: 20mM Tris-HCl (pH 8.0), 1mM DTT, 0.1mM EDTA, 100mM KCl, Stabilizers, and 50%glycerol. 10X Reaction Buffer: 200 mM TrisHCI (pH 8.8)100 mM KCI100 mM (NH4)2SO420 mM Mg SO41% Triton X-1001 mg/ml bovine serum albumin (BSA). Reaction Conditions: DNA synthesis is performed in 100?l of mixture containing 20-200μm dNTPs, 0.3-1μm Promers, 0.1-0.250 mg of template DNA, 10 ?l of 10 x reaction buffer and 2.5-5 units of Taq Plus. Mix the reaction gently, centrifuge briefly and then overlay with light mineral oil. Initially, denature the reaction by incubating at 95? for 5 minutes and then cool to 40-68? for 5 minutes to allow the primers to anneal to the template DNA. Optimization of DNA Synthesis: It is important to ad the reaction components in the following order1. H2O.2. 10 x reaction buffer.3. dNTPs.4. DNA template and primers.5. Taq Plus

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Key Properties

• Source: Recombinant E.coli contains Thermus aquaticus polymerase gene

Storage & Handling

• Storage: Stability: Stable for 5 days at 10°C, for longer period of time store at -20°C
• Buffer/Preservatives: 5U/µl.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Taq Plus DNA Polymerase, Taq Plus DNA, TaqPDNA.