SYNPR/Synaptoporin Rabbit Polyclonal Antibody

• Catalog Number: orb158541
• Category: Antibodies
• Description: SYNPR/Synaptoporin Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Human, Mouse
• Reactivity: Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Synaptoporin (31-130/265aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 29 kDa
• Target: SYNPR
• UniProt ID: Q8TBG9
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: SPO; Synaptophysin 2; Synaptoporin; Synpr; SYNPR_H
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue): RSC96 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-SYNPR/Synaptoporin antibody (orb158541), dilution: 5 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SYNPR) Polyclonal Antibody, Unconjugated (orb158541) at 1:800 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Bulbus olfactorius (Rat) Lysate at 40 ug, Primary: Anti-Synaptoporin (orb158541) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29 kD, Observed band size: 28 kD.