Syndecan-1/CD138 Extracellular domain Antibody

SKU: orb1294301

Description

Rabbit polyclonal antibody to Syndecan-1

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution range: Western Blot 1:500-1:1000 Immunofluorescence 1:200-1:400 Immunohistochemistry (Paraffin) 1:200 - 1:300
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Extracellular domain corresponding to Human CD138 / Syndecan-1
• Target: Syndecan-1 / CD138 Extracellular domain

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

All lanes: anti-Syndecan-1 / CD138 antibody at 1/1000 dilution, Lysates/proteins at 40 µg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294301 overnight at 4°C.

All lanes: anti-Syndecan-1 / CD138 antibody at 1/1000 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294301 overnight at 4°C.

All lanes: anti-Syndecan-1 / CD138 antibody at 1/500 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 5&10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294301 overnight at 4°C.

All lanes: anti-Syndecan-1 / CD138 antibody at 1/500 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 5&10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294301 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294301 anti-CD138 / Syndecan-1 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD138 / Syndecan-1 with orb1294301 at 1/200.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD138 / Syndecan-1 with orb1294301 at 1/200.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD138 / Syndecan-1 with orb1294301 at 1/200.

Quick Database Links

Gene Symbol

Syndecan-1 / CD138 Extracellular domain