Swine IgG (H&L) Antibody Fluorescein Conjugated

• Catalog Number: orb346715
• Category: Antibodies
• Description: Swine IgG (H&L) antibody (FITC)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: FITC
• Reactivity: Porcine
• Form/Appearance: Lyophilized
• Concentration: 10.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Swine IgG and Swine Serum.
• Immunogen: Swine IgG whole molecule
• Tested applications: FC, FLISA, IF, WB
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Application notes: Anti-Swine IgG (H&L) Fluorescein Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: rabbit anti-Swine IgG Fluorescein Conjugated Antib
• Note: For research use only

Western Blot of Fluorescein Conjugated Rabbit anti-Swine IgG antibody. Lane 1: Swine IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Fluorescein swine secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 55 kDa, 28 kDa for Rabbit IgG. Other band(s): none.