SUV420H2 Antibody [RAB-C409], Rabbit IgG

• Catalog Number: orb1671523
• Category: Antibodies
• Description: SUV420H2 Antibody [RAB-C409], Rabbit IgG
• Clonality: Recombinant
• Clone Number: RAB-C409
• Tested applications: ChIP, ELISA
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: This antibody was obtained by recombinant antibody (rAb) phage display recognizing SUV420H2 protein under non-denaturing conditions.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: KMT5C
• UniProt ID: Q86Y97
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Suv4-20h2, Suppressor of variegation 4-20 homolog
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed HEK293 cells with anti-SUV420H2 antibody R Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells on Shi-fix™ coverslips stained with the chimeric r version at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right- top-bottom orb1671523- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Flow cytometry using the anti-SUV420H2 antibody R HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r1 version (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.