Superoxide Dismutase 1/SOD1 Antibody

• Catalog Number: orb27559
• Category: Antibodies
• Description: Superoxide Dismutase 1/SOD1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, WB
• Predicted Reactivity: Monkey
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of Human SOD1 (113-129aa IIGRTLVVHEKADDLGK), different from the mouse sequence by two amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, RatImmunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat, HumanWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 15936 MW
• UniProt ID: P00441
• Sensitivity: < 10pg/ml
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Superoxide dismutase [Cu-Zn];1.15.1.1;Superoxide d
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Immunocytochemical staining of paraffin embedded HELA cell tissue using SOD antibody

Western blot analysis of COLO320 Cell Lysate (Lane1) and SMMC Cell Lysate (Lane2) using SOD antibody

Western blot analysis of Mouse's liver, heart and lung tissue lysates using SOD antibody