You have no items in your shopping cart.
Cart summary
Item 1 of 3
Item 1 of 3
SUMO2/3 Antibody (C-term E69)
Catalog Number: orb1937635
| Catalog Number | orb1937635 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB15398 |
| Tested applications | IF, IHC-P, WB |
| Predicted Reactivity | Hamster, Mouse, Other, Porcine, Rat, Zebrafish |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Dilution range | IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 11637 Da |
| Target | This SUMO2/3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 53-84 amino acids from the C-terminal region of human SUMO2/3. |
| UniProt ID | P55854 |
| NCBI | NP_008867.2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Small ubiquitin-related modifier 3, SUMO-3, SMT3 h Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Fluorescent confocal image of Hela cell stained with SUMO2/3 Antibody (C-term E69). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SUMO2/3 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). SUMO2/3 immunoreactivity is localized to Nucleus significantly.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with SUMO2/3 antibody (C-term E69), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
SUMO2/3 Antibody (C-term E69) western blot analysis in 293, Hela cell line lysates (35 ug/lane). This demonstrates the SUMO2/3 antibody detected the SUMO2/3 protein (arrow).
