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Catalog Number | orb500982 |
---|---|
Category | Antibodies |
Description | Substance P Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC-Fr, IHC-P |
Predicted Reactivity | Bovine, Equine, Porcine, Rabbit, Sheep |
Reactivity | Guinea pig, Human, Mouse, Rat |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human Substance P (RPKPQQFFGLM) (58-68/129aa) |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500, Flow-Cyt=1μg/Test |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 1.4/13 kDa |
Target | TAC1 |
UniProt ID | P20366 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | Hs.2563; Neurokinin 1; Neurokinin 2; Neurokinin A; Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Blank control: Mouse brain cells (blue). Primary Antibody: Rabbit Anti-Substance P antibody (orb500982), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (orb500982, 1 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.
Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Substance P) Polyclonal Antibody, Unconjugated (orb500982) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
U-2OS cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Substance P) polyclonal Antibody, Unconjugated (orb500982) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
ELISA, WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Porcine | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Canine, Equine, Guinea pig, Rabbit, Sheep, Yeast | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |