Streptavidin antibody (Peroxidase)

• Catalog Number: orb344728
• Category: Antibodies
• Description: Streptavidin antibody (Peroxidase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bacteria
• Isotype: IgG
• Immunogen: Streptavidin [Streptomyces avidinii]
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000-1:10,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Streptavidin Antibody Peroxidase Conjugate is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Streptavidin [Streptomyces avidinii]. No cross reactivity occurs against avidin.
• Conjugation: HRP
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: rabbit anti-Streptavidin Antibody HRP Conjugation,
• Note: For research use only
• Application notes: Anti-Streptavidin Peroxidase antibody has been tested by ELISA and western blot. This product has been assayed against 1.0 ug of Streptavidin in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) for 30 minutes at room temperature. Optimal concentrations in immunoassays should be determined by researcher.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of trypsin inhibitor reduced (Lane1), trypsin inhibitor non-reduced (Lane2), Streptavidin reduced (Lane3), Streptavidin non-reduced (Lane4), Load: 1ug per lane using Streptavidin antibody (Peroxidase)