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Catalog Number | orb344727 |
---|---|
Category | Antibodies |
Description | Streptavidin antibody (Peroxidase) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Bacteria |
Isotype | IgG |
Immunogen | Streptavidin [Streptomyces avidinii] |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:2,000-1:10,000 |
Form/Appearance | Lyophilized |
Purity | Streptavidin Antibody Peroxidase Conjugate is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Streptavidin [Streptomyces avidinii]. No cross reactivity occurs against avidin. |
Conjugation | HRP |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
Alternative names | rabbit anti-Streptavidin Antibody HRP Conjugation, Read more... |
Note | For research use only |
Application notes | Anti-Streptavidin Peroxidase antibody has been tested by ELISA and western blot. This product has been assayed against 1.0 ug of Streptavidin in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) for 30 minutes at room temperature. Optimal concentrations in immunoassays should be determined by researcher. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of trypsin inhibitor reduced (Lane1), trypsin inhibitor non-reduced (Lane2), Streptavidin reduced (Lane3), Streptavidin non-reduced (Lane4), Load: 1ug per lane using Streptavidin antibody (Peroxidase)
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