STK40 Rabbit Polyclonal Antibody

SKU: orb101780

Description

STK40 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Human, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human STK40 (231-330/435aa)
• Target: STK40
• Molecular Weight: 48 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Ser/Thr like kinase; Serine/threonine kinase 40; Serine/threonine protein kinase 40; SgK495; SHIK; SINK homologous serine/threonine protein kinase; SINK-homologous serine/threonine-protein kinase; STK 40; Sugen kinase 495; STK40_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (STK40) Polyclonal Antibody, Unconjugated (orb101780) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Rat Cerebrum tissue lysates, Primary: Anti-STK40 (orb101780) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kDa, Observed band size: 48 kDa.

Quick Database Links

Gene Symbol

STK40

UniProt

Q8N2I9

References

Xia Li # 1 2, Li-Zhen Shao # 1 2, Zhuo-Hang Li 2 3, Yong-Heng Wang 1 2, Qin-Yu Cai 1 2, Shun Wang 1, Hong Chen 1 2, Jie Sheng 1 2, Xin Luo 2 4, Xue-Mei Chen 2, Ying-Xiong Wang 5, Yu-Bin Ding 6, Tai-Hang Liu STK40 inhibits trophoblast fusion by mediating COP1 ubiquitination to degrade P57Kip2 J Transl Med, (2024)

PubMed: 39304928

Applications

IF

Reactivity

Human

Fong, Louise Y. et al. Abrogation of esophageal carcinoma development in miR-31 knockout rats Proc. Natl. Acad. Sci. U.S.A., 117, 6075-6085 (2020)

PubMed: 32123074