STK39 Antibody

• Catalog Number: orb1249610
• Category: Antibodies
• Description: STK39 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen for this antibody is: C-SQEKSRRVKEENPE
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 65kDa band observed in Human Brain (Cerebellum) lysates and in lysates of cell line Jurkat (calculated MW of 59.5kDa according to NP_037365.2). Approx 75kDa band observed in Mouse and Rat Brain lysates (calculated MW of 60.3kDa according to Mouse NP_058562.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows textured staining in the neurons. Recommended concentration, 3-5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: STK39
• UniProt ID: Q9UEW8
• NCBI: NP_037365.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: STK39, serine threonine kinase 39 (STE20/SPS1 homo
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 65kDa band observed in Human Brain (Cerebellum) lysates and in lysates of cell line Jurkat (calculated MW of 59.5kDa according to NP_037365.2). Approx 75kDa band observed in Mouse and Rat Brain lysates (calculated MW of 60.3kDa according to Mouse NP_058562.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows textured staining in the neurons. Recommended concentration, 3-5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249610 (0.5 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249610 (3.8 ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1249610 (1 ug/ml) staining of Mouse (A) and Rat (B) Brain (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.