STK39 Antibody

SKU: orb1249610

Description

STK39 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 65kDa band observed in Human Brain (Cerebellum) lysates and in lysates of cell line Jurkat (calculated MW of 59.5kDa according to NP_037365.2). Approx 75kDa band observed in Mouse and Rat Brain lysates (calculated MW of 60.3kDa according to Mouse NP_058562.1). Recommended concentration: 0.3-1µg/mL.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows textured staining in the neurons. Recommended concentration, 3-5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-SQEKSRRVKEENPE
• Target: STK39
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

STK39, serine threonine kinase 39 (STE20/SPS1 homolog, yeast), DCHT, DKFZp686K05124, PASK, SPAK, Ste20-like protein kinase, proline-alanine-rich STE20-related kinase, small intestine SPAK-like kinase

orb1249610 (0.5 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249610 (3.8 ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1249610 (1 ug/ml) staining of Mouse (A) and Rat (B) Brain (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

STK39

UniProt

Q9UEW8

RefSeq (Protein):NP_037365.2