STK35 antibody

• Catalog Number: orb18928
• Category: Antibodies
• Description: Goat polyclonal antibody to STK35
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:16000, WB: 1-3 μg/ml, IHC-P: 10 μg/ml
• Conjugation: Unconjugated
• MW: 58.1
• Target: Clik1 / STK35
• Entrez: 140901
• Protein Sequence: ELETRMDQVTCAA
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti STK35 antibody, anti Clik1 antibody, anti bA5
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded human testis shows granular cytoplasmic staining of Leydig cells. Recommended concentration, 10ug/ml.WB: Approx 45kDa band observed in human testis lysates (calculated MW of 44.6kDa according to NP_543026. Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb18928 (1ug/ml) staining of human testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb18928 (10ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

WB analysis of Mouse (A) and Pig (B) Testes lysate using STK35 antibody

Immunofluorescence analysis of U2OS cells using STK35 antibody

Flow cytometric analysis of HEK293 cells using STK35 antibody