STK18 Rabbit Polyclonal Antibody

SKU: orb1974732

Description

STK18 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=2ug/Test
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PLK4/STK18 (31-130/970aa)
• Target: PLK4
• Molecular Weight: 108 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

PLK 4; PLK-4; PLK4; PLK4_HUMAN; Polo like Kinase 4; Polo-like kinase 4; Protein serine/threonine kinase; SAK; Serine/Threonine Kinase 18; Serine/threonine kinase; Serine/threonine protein kinase 18; Serine/threonine protein kinase PLK4; Serine/threonine protein kinase Sak; Serine/threonine-protein kinase 18; Serine/threonine-protein kinase PLK4; Serine/threonine-protein kinase SAK; Snk Akin Kinase; STK 18.

Blank control (black line): MCF7. Primary Antibody (green line): Rabbit Anti-STK18 antibody (orb1974732), dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (STK18) polyclonal Antibody, Unconjugated (orb1974732) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Lane 1: Human MCF-7 cell lysates, Lane 2: Human HeLa cell lysates, Primary: Anti-STK18 (orb1974732) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 108 kDa, Observed band size: 110 kDa.

Quick Database Links

Gene Symbol

PLK4

UniProt

O00444