Cart summary

You have no items in your shopping cart.

STK18 Rabbit Polyclonal Antibody

Catalog Number: orb1974732

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb1974732
CategoryAntibodies
DescriptionSTK18 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, ICC, WB
Predicted ReactivityBovine, Canine, Equine, Mouse, Porcine, Rabbit, Rat
ReactivityHuman
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human PLK4/STK18 (31-130/970aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, ICC/IF=1:25, Flow-Cyt=2ug/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW108 kDa
TargetPLK4
UniProt IDO00444
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesPLK 4; PLK-4; PLK4; PLK4_HUMAN; Polo like Kinase 4
Read more...
NoteFor research use only
Expiration Date12 months from date of receipt.
STK18 Rabbit Polyclonal Antibody

Blank control (black line): MCF7. Primary Antibody (green line): Rabbit Anti-STK18 antibody (orb1974732), dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

STK18 Rabbit Polyclonal Antibody

MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (STK18) polyclonal Antibody, Unconjugated (orb1974732) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

STK18 Rabbit Polyclonal Antibody

Sample: Lane 1: Human MCF-7 cell lysates, Lane 2: Human HeLa cell lysates, Primary: Anti-STK18 (orb1974732) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 108 kDa, Observed band size: 110 kDa.

  • Anti-SAK Antibody [orb412909]

    WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 200 μl, 50 μl
  • PLK4 Antibody [orb630014]

    ELISA,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μg, 50 μg
  • PLK4/STK18 Rabbit Polyclonal Antibody [orb559042]

    IF,  IHC-Fr,  IHC-P

    Bovine, Mouse, Rabbit, Rat, Sheep

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 200 μl, 50 μl
  • PLK4/STK18 Rabbit Polyclonal Antibody [orb608044]

    IF,  IHC-Fr,  IHC-P

    Bovine, Canine, Mouse, Rabbit, Rat, Sheep

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl, 200 μl
  • PLK4 Antibody (C-Term) [orb1926159]

    WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl