STING1 Rabbit Polyclonal Antibody

• Catalog Number: orb1229
• Category: Antibodies
• Description: STING1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Human
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human STING1 (231-330/379aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 42 kDa
• Target: STING1
• UniProt ID: Q86WV6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Endoplasmic reticulum interferon stimulator; Media
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TMEM173) Polyclonal Antibody, Unconjugated (orb1229) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: Mouse Lung tissue lysates, Lane 2: Mouse Uterus tissue lysates, Lane 3: Mouse Thymus tissue lysates, Lane 4: Mouse Large intestine tissue lysates, Lane 5: Rat Lung tissue lysates, Lane 6: Rat Uterus tissue lysates, Lane 7: Rat Thymus tissue lysates, Lane 8: Rat Large intestine tissue lysates, Primary: Anti-STING1 (orb1229) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42 kDa, Observed band size: 38 kDa.