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    STING antibody

    Catalog Number: orb224150

    DispatchUsually dispatched within 5-10 working days
    $ 337.00
    Catalog Numberorb224150
    CategoryAntibodies
    DescriptionRabbit polyclonal antibody to STING
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsIF, IH, WB
    ReactivityHuman
    ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human STING. The exact sequence is proprietary.
    Dilution rangeWB: 1:500-1:1000, IP: 1:10-1:100
    Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    ConjugationUnconjugated
    TargetTMEM173
    Entrez340061
    UniProt IDQ86WV6
    SourceRabbit
    StorageShipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
    Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    Alternative namesanti ERIS antibody, anti MITA antibody, anti STING
    Read more...
    NoteFor research use only
    Expiration Date12 months from date of receipt.
    STING antibody

    Western blot analysis of STING expression in HEK293 (A), Hela (B) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 33 kD)

    STING antibody

    Immunohistochemical analysis of STING staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    STING antibody

    Immunofluorescent analysis of STING staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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