STAT5b Antibody

• Catalog Number: orb308807
• Category: Antibodies
• Description: STAT5b Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human STAT5b (675-703aa KDEVYSKYYTPVPCESATAKAVDGYVKPQ), different from the related mouse and rat sequences by two amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 89866 MW
• UniProt ID: P51692
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Signal transducer and activator of transcription 5
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of Jurkat cells using anti-STAT5b antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of A431 cells using anti-STAT5b antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of STAT5b using anti-STAT5b antibody.Lane 1:K562 Cell;2:HEK293 Cell;3:PANC-1 Cell;4:SW620 Cell;5:SGC-7901 Cell;6:Rat Lung Tissue.7:Rat Liver Tissue.8:Mouse Spleen Tissue.9:Mouse Spleen Tissue.10:Mouse Lung Tissue.

IF analysis of STAT5b using anti-STAT5b antibody. STAT5b was detected in immunocytochemical section of U20S cells.