STAT5A antibody

• Catalog Number: orb176732
• Category: Antibodies
• Description: Goat polyclonal antibody to STAT5A
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC, WB
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:8000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 90.6
• Target: STAT5A (aa681-692)
• Entrez: 6776
• Protein Sequence: KYYTPVLAKAVD
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti MGF antibody, anti signal transducer and acti
• Note: For research use only
• Application notes: WB: Approx 100kDa band observed in lysates of cell line K562 (calculated MW of 90.6kDa according to NP_003143.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of K562 lysate using STAT5A antibody