STAT5a Antibody

• Catalog Number: orb1524731
• Category: Antibodies
• Description: STAT5a Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: between 744 and 794
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: STAT5a
• UniProt ID: P42229
• NCBI: NP_003143.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: signal transducer and activator of transcription 5
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse STAT5a by western blot. Samples: Whole cell lysate from HeLa (15 and 50 µg) , HEK293T (T; 50 µg) , Jurkat (J; 50 µg) , and mouse NIH 3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-STAT5a antibody orb1524731 used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human STAT5a by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-STAT5a antibody orb1524731 used for IP at 6 µg/mg lysate.

Detection of human STAT5a by immunohistochemistry.Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-STAT5a (Cat. No. orb1524731) used at a dilution of 1: 200 (1µg/ml). Detection: DAB