STAT5 antibody

• Catalog Number: orb345603
• Category: Antibodies
• Description: STAT5 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near the carboxy terminus of mouse Stat5a protein.
• Concentration: 1.2 mg/mL
• Dilution range: ELISA: 1:25,000, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using peptide coupled to agarose beads. This antibody is specific for mouse Stat5 protein. A BLAST analysis was used to suggest cross-reactivity with Stat5 from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Stat5 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P42230
• NCBI: 6755672
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-STAT5 antibody, STAT-5, STAT 5, STAT5A
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kDa in size corresponding to phosphorylated Stat5 protein by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HeLa cell extract (arrowhead) using STAT5 antibody