STAT4 Rabbit Polyclonal Antibody

SKU: orb158505

Description

STAT4 Rabbit Polyclonal Antibody

Research Area

JAK/ STAT Signaling Pathway

Images & Validation

• Tested Applications: ELISA, ICC, WB
• Dilution range: WB=1:500-2000, ICC/IF=1:100-500, ELISA=1:5000-10000
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human STAT4 (111-210/748aa)
• Target: STAT4
• Molecular Weight: 85 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

signal transducers and activators of transduction 4; CIS4; HSPC060; SOCS4; SOCS6; SSI4; STAI4; STAT 4; STAT4; STATI4; Suppressor of cytokine signaling 6; STAT4_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (STAT4) Polyclonal Antibody, Unconjugated (orb158505) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

STAT4

UniProt

Q14765