STAT3 Rabbit Polyclonal Antibody

SKU: orb499707

Description

STAT3 Rabbit Polyclonal Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human STAT3 (151-250/770aa)
• Target: STAT3
• Molecular Weight: 88 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ADMIO; ADMIO1; APRF; HIES; 1110034C02Rik; STAT3_HUMAN; STAT3; Acute-phase response factor; STAT3_MOUSE; STAT3_RAT;

Paraformaldehyde-fixed, paraffin embedded (Human breast cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (STAT3) Polyclonal Antibody, Unconjugated (orb499707) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Sample: RSC96 cells (pre-warmed 4% paraformaldehyde was added and the cells were incubated for 10 min at 37°C), Primary Antibody: Rabbit Anti-STAT3 antibody (orb499707) (green), Concentration: 0.2 µg/10^6 cells, Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: F (ab')2 fragment goat anti-rabbit IgG-PE (white blue).

Quick Database Links

Gene Symbol

STAT3

UniProt

P40763