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Catalog Number | orb345727 |
---|---|
Category | Antibodies |
Description | STAT3 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | DOT, ELISA, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | STAT3 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with synthetic monomethylated or asymmetric dimethylated peptides surrounding Arginine 31 of human STAT. |
Concentration | 1.0 mg/ml |
Dilution range | ELISA: 1:5000, WB: 1:500 |
Form/Appearance | Liquid (sterile filtered) |
Purity | Anti-STAT3 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with all STAT3 modifications. Cross-reactivity is seen against STAT1 and STAT4. BLAST analysis was used to suggest cross-reactivity with Human, mouse, and rat. Cross-reactivity with STAT3 from other sources has not been determined. |
Conjugation | Unconjugated |
UniProt ID | P40763 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.05% (w/v) Sodium Azide |
Alternative names | rabbit anti-STAT3 antibody, STAT-3, STAT 3, Signal Read more... |
Note | For research use only |
Application notes | Anti-STAT3 antibody has been tested by Dot Blot and is useful for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~88 kDa corresponding to STAT3 protein by Western Blotting in the appropriate cell lysate or extract. |
Expiration Date | 12 months from date of receipt. |
Dot Blot analysis of monomethylated and asymmetric dimethylated forms of the immunizing peptide using STAT3 antibody
FC, IHC-P, WB | |
Bovine, Canine, Guinea pig, Porcine, Rabbit, Sheep | |
Gallus, Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, IF, IHC | |
Bovine, Human, Mouse, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
IHC, IP, WB | |
Human, Mouse, Rat | |
Unconjugated |
FC, ICC, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
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