STAT3 (phospho-Ser727) antibody

• Catalog Number: orb500068
• Category: Antibodies
• Description: STAT3 (phospho-Ser727) antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: 4G1
• Tested applications: FC, ICC, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human STAT3 around the phosphorylation site of Ser727 (PM(p-S)PR)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-200, ICC=1:50-100, Flow-Cyt=2ug/Test, IHC-F=1:100-200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 88 kDa
• Target: phospho-STAT3 (Ser727)
• UniProt ID: P40763
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: STAT3 (phospho S727); p-STAT3 (phospho S727); STAT
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry of A431.Primary Antibody (green line): Rabbit Anti-phospho-STAT3 (Ser727) antibody; Dilution:1μg /10^6 cells.

ICC analysis of A431 cell using phospho-STAT3 (Ser727) monoclonal Antibody at 1:100 dilution;DAPI (blue) was used to stain the cell nuclei.

IHC-P analysis of rat brain using phospho-STAT3 Ser727 Polyclonal Antibody at 1:200 dilution.

Western blot:Heart (Rat);Cerebrum (Rat);Primary:Anti-phospho-STAT3 (Ser727) at 1:1000 dilution.

Western blot:Lane 1:Hela (Human) Cell;Lane 2:A549 (Human) Cell;Primary:Anti-phospho-STAT3 (Ser727) at 1:1000 dilution.