STAT3 Antibody

• Catalog Number: orb1274529
• Category: Antibodies
• Description: STAT3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 640-770 of human STAT3 (NP_644805.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 90kDa
• Target: STAT3
• UniProt ID: P40763
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: STAT3 Antibody: APRF, HIES, APRF, Signal transduce
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using STAT3 antibody (orb1274529) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Western blot analysis of extracts from normal (control) and STAT3 knockout (KO) HeLa cells, using STAT3 antibody (orb1274529) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunofluorescence analysis of rat brain using STAT3 antibody (orb1274529) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of mouse brain using STAT3 antibody (orb1274529) at dilution of 1:100. Blue: DAPI for nuclear staining.