STAT3 Antibody

• Catalog Number: orb1253817
• Category: Antibodies
• Description: STAT3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: STAT3 (Phospho-Ser727) antibody was raised against a peptide sequence around phosphorylation site of serine 727 (P-M-S (p) -P-R) derived from Human STAT3.
• Concentration: 1 mg/mL
• Dilution range: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 88 kDa
• Target: STAT3
• UniProt ID: P40763
• NCBI: NP_003141.2
• Storage: Store antibody at -20°C for up to one year.
• Buffer/Preservatives: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Alternative names: APRF, HIES, STAT5, APRF, Acute-phase response fact
• Note: For research use only
• Application notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysed extracts from HeLa and HepG2 cells untreated or treated with PMA using STAT3 (Phospho-Ser727).

Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT3 (Phospho-Ser727). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using STAT3 (Phospho-Ser727).