StAR Rabbit Polyclonal Antibody

• Catalog Number: orb7014
• Category: Antibodies
• Description: StAR Rabbit Polyclonal Antibody
• Target: STAR
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human StAR/StARD1 (101-200/285aa)
• UniProt ID: P49675
• RRID: AB_10941117
• MW: 32 kDa
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=3μg /Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: StARD1; Cholesterol trafficker; Luteinizing hormon
• Note: For research use only

References

Rore, Holly et al. Testicular somatic cell-like cells derived from embryonic stem cells induce differentiation of epiblasts into germ cells Commun Biol, 4, 802 (2021)

PubMed: 34183774

Blank control (blue line): Hela (fixed with 80% methanol (5 min at -20°C) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-StAR antibody (orb7014), dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

Paraformaldehyde-fixed, paraffin embedded (Rat testis), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (StAR) Polyclonal Antibody, Unconjugated (orb7014) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Sample: Lane 1: Ovary (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Lane 3: Kidney (Mouse) Lysate at 40 ug, Lane 4: Cerebrum (Rat) Lysate at 40 ug, Lane 5: K562 (Human) Cell Lysate at 30 ug, Lane 6: THP-1 (Human) Cell Lysate at 30 ug, Lane 7: U937 (Human) Cell Lysate at 30 ug, Lane 8: HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-StAR (orb7014) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 32 kD, Observed band size: 28 kD.