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Catalog Number | orb158499 |
---|---|
Category | Antibodies |
Description | Stabilin 2 Rabbit Polyclonal Antibody |
Target | STAB2 |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Mouse |
Predicted Reactivity | Bovine, Human, Rabbit, Rat, Sheep |
Form/Appearance | Liquid |
Concentration | 1mg/ml |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Immunogen | KLH conjugated synthetic peptide derived from human Stabilin 2 (2201-2300/2551aa) |
UniProt ID | Q8WWQ8 |
MW | 154/275 kDa |
Tested applications | IF, IHC-Fr, IHC-P |
Dilution range | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | STAB 2; 190 kDa form stabilin-2; 190 kDa hyalurona Read more... |
Note | For research use only |
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Fajardo, Val A. et al. Effects of sarcolipin deletion on skeletal muscle adaptive responses to functional overload and unload Am J Physiol Cell Physiol, 313, C154-C161 (2017)
Applications
Reactivity
Fajardo, Val A. et al. Sarcolipin deletion in mdx mice impairs calcineurin signalling and worsens dystrophic pathology Hum Mol Genet, 27, 4094-4102 (2018)
Applications
Reactivity
Fajardo, Val A. et al. Sarcolipin deletion exacerbates soleus muscle atrophy and weakness in phospholamban overexpressing mice PLoS One, 12, e0173708 (2017)
Applications
Reactivity
Tissue/Cell: mouse spleen tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Stabilin 2 Polyclonal Antibody, Unconjugated (orb158499) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF | |
Bovine, Human, Rabbit, Rat, Sheep | |
Mouse | |
Rabbit | |
Polyclonal | |
Cy3 |
IF | |
Bovine, Human, Rabbit, Rat, Sheep | |
Mouse | |
Rabbit | |
Polyclonal | |
PerCP/Cy5.5 |
IF | |
Bovine, Human, Rabbit, Rat, Sheep | |
Mouse | |
Rabbit | |
Polyclonal | |
PerCP |
IF | |
Bovine, Human, Rabbit, Rat, Sheep | |
Mouse | |
Rabbit | |
Polyclonal | |
BF750 |