Stabilin 2 Rabbit Polyclonal Antibody

SKU: orb158499

Description

Stabilin 2 Rabbit Polyclonal Antibody

Research Area

Endothelial, Molecular Biology, Surface Molecules, Varscular System

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Human, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Stabilin 2 (2201-2300/2551aa)
• Target: STAB2
• Molecular Weight: 154/275 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

STAB 2; 190 kDa form stabilin-2; 190 kDa hyaluronan receptor for endocytosis; EGF-like; FAS1 EGF-like and X-link domain-containing adhesion molecule 2; Fasciclin; FEEL-2; FEEL2; FELL; FEX2; HARE; Hyaluronan receptor for endocytosis; laminin-type EGF-like and link domain-containing scavenger receptor 2; link domain-containing scavenger receptor 2; STAB-2; Stab2; STAB2_HUMAN.

Tissue/Cell: mouse spleen tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Stabilin 2 Polyclonal Antibody, Unconjugated (orb158499) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

STAB2

UniProt

Q8WWQ8

References

Fajardo, Val A. et al. Effects of sarcolipin deletion on skeletal muscle adaptive responses to functional overload and unload Am J Physiol Cell Physiol, 313, C154-C161 (2017)

PubMed: 28592414

Applications

WB

Reactivity

Mouse

Fajardo, Val A. et al. Sarcolipin deletion in mdx mice impairs calcineurin signalling and worsens dystrophic pathology Hum Mol Genet, 27, 4094-4102 (2018)

PubMed: 30137316

Applications

WB

Reactivity

Mouse

Fajardo, Val A. et al. Sarcolipin deletion exacerbates soleus muscle atrophy and weakness in phospholamban overexpressing mice PLoS One, 12, e0173708 (2017)

PubMed: 28278204

Applications

WB

Reactivity

Mouse