ST18 Rabbit Polyclonal Antibody

• Catalog Number: orb158498
• Category: Antibodies
• Description: ST18 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Canine, Equine, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ST18/Znf387 (451-550/1047aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=3ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 115 kDa
• Target: ST18
• UniProt ID: O60284
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: KIAA0535; St18; ST18_HUMAN; suppression of tumorig
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: Mouse kidney. Primary Antibody (green line): Rabbit Anti-ST18 antibody (orb158498), dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-ST18 (orb158498) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 115 kD, Observed band size: 110 kD.

Sample: U87MG (Human) Cell Lysate at 30 ug, Primary: Anti-ST18 (orb158498) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 115 kD, Observed band size: 115 kD.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-ST18 Polyclonal Antibody, Unconjugated (orb158498) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.