SSH3BP1/ABI1 Antibody

• Catalog Number: orb259593
• Category: Antibodies
• Description: SSH3BP1/ABI1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human ABI1 (151-175aa HGVKWLKAKHGNNQPARTGTLSRTN), identical to the related mouse and rat sequences.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry, 1-3 μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 55081 MW
• UniProt ID: Q8IZP0
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Abl interactor 1;Abelson interactor 1;Abi-1;Abl-bi
• Note: For research use only
• Application notes: WB: The detection limit for ABI1 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of K562 cells using anti-SSH3BP1/ABI1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of SSH3BP1/ABI1 using anti-SSH3BP1/ABI1 antibody.Lane 1:human 293T cell;2:human MCF-7 cell;3:human U87 cell;4:rat brain tissue;5:rat C6 cell;6:mouse brain tissue.

IF analysis of SSH3BP1/ABI1 using anti-SSH3BP1/ABI1 antibody. SSH3BP1/ABI1 was detected in an immunocytochemical section of A431 cells.

IHC analysis of SSH3BP1/ABI1 using anti-SSH3BP1/ABI1 antibody. SSH3BP1/ABI1 was detected in a paraffin-embedded section of mouse brain tissue.

IHC analysis of SSH3BP1/ABI1 using anti-SSH3BP1/ABI1 antibody. SSH3BP1/ABI1 was detected in a paraffin-embedded section of rat brain tissue.