SSB1 Antibody

• Catalog Number: orb1527524
• Category: Antibodies
• Description: SSB1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 161 and 211
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for human tissues. Epitope retrieval with citrate buffer pH 6.0 is recommended for mouse tissues.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: SSB1
• UniProt ID: Q9BQ15
• NCBI: NP_076973.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: oligonucleotide/oligosaccharide-binding fold-conta
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human SSB1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB) and HEK293T (T; 50 µg for WB; 1 mg for IP, 20% of IP loaded) cells. Antibody: Affinity purified rabbit anti-SSB1 antibody orb1527524 used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 5 seconds (B).

Detection of human and mouse SSB1 by immunohistochemistry.Sample: FFPE sections of human colon carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-SSB1 (Cat. No. orb1527524) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB