SRXN1 Rabbit Polyclonal Antibody

• Catalog Number: orb2565
• Category: Antibodies
• Description: SRXN1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human SRXN1 (45-137/137aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 15 kDa
• Target: SRXN1
• UniProt ID: Q9BYN0
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: C20orf1392; Chromosome 20 open reading frame 139;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by microwave in sodium citratebuffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RTfor 30 min, Antibody incubation with (SRXN1) Polyclonal/MonoclonalAntibody, Unconjugated (orb2565) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Sample: Kidney (Mouse) Lysate at 40 ug, Primary: Anti-SRXN1 (orb2565) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 15 kD, Observed band size: 15 kD.

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Rat) Lysate at 40 ug, Lane 3: HepG2 (Human) Cell Lysate at 30 ug, Lane 4: A549 (Human) Cell Lysate at 30 ug, Primary: Anti-SRXN1 (orb2565) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 15 kD, Observed band size: 15 kD.